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HPLC Column Void Volume table
 ÀÛ¼ºÀÚ : ÃæÀΰúÇÐ
Date : 2015-07-28 17:03  |  Hit : 1,332  

                HPLC Column VOLUME TABLE


   COLUMN DIMENSIONS             VOID VOLUME (ml)
        ( I.D. x L(mm) )      
      
         1.0     x     100                            0.06  
         1.0     x     150                            0.08
         1.0     x     250                            0.14  
         1.0     x     300                            0.17

         2.1     x     100                            0.24
         2.1     x     150                            0.37
         2.1     x     250                            0.61
         2.1     x     300                            0.73

         4.6     x     100                            1.16  
         4.6     x     150                            1.75
         4.6     x     250                            2.90
         4.6     x     300                            3.49

         7.8     x     100                            3.35
         7.8     x     150                            5.02
         7.8     x     250                            8.36
         7.8     x     300                           10.04

        10.0     x     100                            5.50
        10.0     x     150                            8.25
        10.0     x     250                           13.75
        10.0     x     300                           16.49

        21.0     x     100                           24.25
        21.0     x     150                           36.37
        21.0     x     250                           60.61
        21.0     x     300                           72.74

        50.0     x     100                          137.45
        50.0     x     150                          206.17
        50.0     x     250                          343.61
        50.0     x     300                          412.33


lNote :  Assumes an Average Pore Volume of 0.70



ÀϹÝÀûÀ¸·Î, Void VolumeÀ» °è»êÇÏ´Â ¹æ¹ýÀº ´ÙÀ½°ú °°´Ù.

*  Void Volume (mL)  =  d2  x  Pi  x  L  x  Pore Volume / 4

¿¹¸¦ µé¾î, Ä÷³ size°¡ 4.6 x 150mm ÀÌ°í  Pore VolumeÀº ´ë·« 0.7mL/g Á¤µµ¶ó¸é,

*   Void Volume (mL)  =   (4.6)2 x 3.14 x 150 x 0.7 / 4
                                =   1744.11 mm
                                =   1.744 mL                  ÀÌ´Ù.

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